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DMEM, Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM). DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for cultivation of certain cell types.

 

 

 

 

 


 

 

Serum gamma irradiation: Gamma irradiation is recognized as a minimally intrusive, effective and reproducible method of inactivating viruses and other adventitious agents in serum. Even though Fetal Bovine Serum is stringently tested, low levels of common bovine viruses below the detection limits of current test methods may exist. Serox brand Gamma Irradiated Fetal Bovine Serum is exposed to a Cobalt-60 source at a delivered dose of 25-35 kGy under tightly controlled conditions. Exposure of serum to this dosage has been shown to remove up to 6 logs of many extraneous agents while preserving the growth performance characteristics of serum.